FIGURE

Fig. 5

ID

ZDB-FIG-221119-31

Publication

Coffey et al., 2021 - Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix

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Fig. 5

The laminin receptor integrin ?7 is not required for laminin-111 protein re-expression at 3.5 dpf in spns1^?/? larvae but does contribute to muscle resilience in spns1^?/? larvae. (A?D) Anterior left, dorsal top, side-mounted larvae. Phalloidin (white) and laminin-111 (cyan) stained WT, itga7^?/?, spns1^?/?, and spns1^?/?; itga7^?/? larvae at 3.5 dpf. (A?D) Merged phalloidin and laminin-111 channels and (A1?D1) single laminin-111 channel. White arrows indicate the MTJ. Red arrowheads point to dystrophic lesions. Yellow star indicates aberrant laminin-111 surrounding detached fibers. (E) Percent of myotomes with dystrophic lesions. Note the significant increase in muscle degeneration in spns1^?/?; itga7^?/? larvae compared to itga7^?/? single mutants. * p < 0.05, **** p < 0.0001. Scale bar 50 �m.

Expression Data

Antibody:	Ab1-lam
Fish:	WT itga7^mai18/mai18 spns1^{hi891Tg/hi891Tg} itga7^mai18/mai18; spns1^{hi891Tg/hi891Tg}
Anatomical Term:	vertical myoseptum muscle tendon junction
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga7^mai18/mai18 spns1^{hi891Tg/hi891Tg} itga7^mai18/mai18; spns1^{hi891Tg/hi891Tg}
Observed In:	myotome skeletal muscle cell vertical myoseptum muscle tendon junction
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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