Normal initial muscle development is followed by abnormal 3.5 dpf laminin-111 levels in spns1?/? embryos and larvae. (A1?H3) Anterior left, dorsal top, side-mounted embryos and larvae stained with phalloidin (white) to visualize actin and laminin-111 antibodies (cyan). (A1?H1) Phalloidin staining. (A2?H2) Laminin-111 staining. (A3?H3) Merged phalloidin and laminin-111 channels. White arrowheads point to laminin-111 localized to the MTJ. Laminin-111 staining, not detected in WT embryos and larvae at 3?3.5 dpf, is detected in (H2?H3) spns1?/? 3.5 dpf larvae. (I,J) Relative fluorescent intensity of laminin-111 protein in WT and spns1?/? embryos and larvae over time (see methods). * p < 0.05, ** p < 0.01, **** p < 0.0001. Scale bars 50 µm.
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