Figure 2

Loss of laminin reduces KRas^V12 cell trans-differentiation into mesenchymal phenotypes

(A and B) Sample images of neural tube cells expressing HRas-EGFP in control (A) and lamc1 MO-injected (B) embryos, stained with DRAQ5 and GFP to quantify cell density in C, from 9 control embryos and 6 lamc1 morphants.

(D–G) Images of EGFP-KRas^V12-expressing invaded cells in controls (D, E) and lamc1 mutants (F, G) stained with GFP and N-cadherin, where the dotted lines outline cells of interest, quantified in H and I.

(H) Number of invaded cells per embryo in control (n = 11) and lamc1 mutant (n = 11) embryos.

(I) Percentage of KRas^V12-invaded cells expressing N-cadherin in controls (n = 11) and lamc1 mutants (n = 11).

(J and K) Examples of neuron-like morphology cells expressing EGFP-KRas^V12 in control (J) and lamc1 mutants (I), where arrowheads denote cell bodies, quantified in l.

(L) Percentage of KRas^V12-invaded cells with neuron-like morphologies in control (n = 11) and lamc1 mutants (n = 11). In A, B, D–G, J, K, scale bars represent 20 μm. In C, H, I, L, the error bars indicate the SD, the box the SEM, and the lines, the mean. n.s., not significant, ∗p < 0.05, ∗∗p < 0.01. See also Figure S1.