Figure 5

RNA-seq analysis of the gene expression profiles in the anterior (S1), middle (S2) and posterior regions (S3) of the intestine at 5 dpf. (A) Graph showing the region covered by the S1, S2 and S3 intestine fragments. (B) Hierarchical clustering heatmap comparing the 452 S1-enriched, 309 S2-enriched and 2449 S3-enriched DEGs among the three S1, S2 and S3 RNA-seq samples at 5 dpf. (C) Hierarchical clustering heatmap showing the distribution of 385 intestine-specific genes within the three S1, S2 and S3 RNA-seq samples at 5dpf. (D–G) WISH showing the expression of representative genes in the regions of S1 (intestinal bulb) (D), S1 + S2 (bulb + mid-intestine) (E), S3 (hindgut) (F) and S1+S2+S3 (entire intestinal tube) (G). (H–J) GO analysis of the 452 S1-enriched (H), 309 S2-enriched (I) and 2449 S3-enriched (J) genes under the GO_BP and GO_MF terms. The top 5 categories are provided. (K) Information for the 8 S1-enriched, 1 S2-enriched and representatives of the 77 S3-enriched TF genes identified by RNA-seq.