FIGURE

Fig. 3.

ID

ZDB-FIG-221029-3

Publication

Venuto et al., 2022 - Alone in a crowd: effect of a nonfunctional lateral line on expression of the social hormone parathyroid hormone 2

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Fig. 3.

Social isolation further reduces pth2 expression in sensory-hair-cell mutants. (A-C) mRNA ISH showing representative examples of pth2-positive cells in 4 dpf (A) socially-reared lhfpl5b^vo35 mutants (n=48), (B) isolated WT larvae (n=50), and (C) isolated lhfpl5b^vo35 mutants (n=40). Scale bar: 50 µm, applies to all images. (D) Boxplots of qPCR results comparing pth2 expression in socially reared lhfpl5b^vo35 and tomt^tk256c mutants relative to isolated mutant and WT siblings at 4 dpf. Ten larval heads were pooled per condition to make one biological replicate, and three biological replicates were used to generate the data. Two technical replicates of the qPCR experiment were conducted per biological replicate and averaged. Two-way ANOVA with Tukey post-hoc test to assess significance, ***P<0.001, *P<0.05; ns, not significant.

Expression Data

Gene:	pth2
Fish:	WT lhfpl5b^vo35/vo35
Condition:	housing conditions
Anatomical Term:	thalamus
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT lhfpl5b^vo35/vo35
Condition:	housing conditions
Observed In:	thalamus
Stage:	Day 4

Phenotype Detail

Acknowledgments

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