ZFIN Image: Venuto et al., 2022, Fig. 3.

Image

Figure Caption

Fig. 3.

Social isolation further reduces pth2 expression in sensory-hair-cell mutants. (A-C) mRNA ISH showing representative examples of pth2-positive cells in 4 dpf (A) socially-reared lhfpl5b^vo35 mutants (n=48), (B) isolated WT larvae (n=50), and (C) isolated lhfpl5b^vo35 mutants (n=40). Scale bar: 50 µm, applies to all images. (D) Boxplots of qPCR results comparing pth2 expression in socially reared lhfpl5b^vo35 and tomt^tk256c mutants relative to isolated mutant and WT siblings at 4 dpf. Ten larval heads were pooled per condition to make one biological replicate, and three biological replicates were used to generate the data. Two technical replicates of the qPCR experiment were conducted per biological replicate and averaged. Two-way ANOVA with Tukey post-hoc test to assess significance, ***P<0.001, *P<0.05; ns, not significant.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open