FIGURE 7

In vivo validation of the involvement of nfkbiaa, perp and mgll in zebrafish early development. (A–C) qRT-PCR analysis of nfkbiaa(A), perp(B) and mgll(C) expression during the course of early embryonic development from unfertilized embryo to 10 hpf. (D) Quantitative analysis of homozygous, heterozygous and wild-type embryos revealed that the expression level of nfkbiaa, perp and mgll was overexpressed in miR-202 homozygous mutant. (E) Time-matched bright field images of slow-developing embryos (top) and the rate of mortality and developmental abnormality (bottom) when extra mRNA of nfkbiaa, perp and mgll were introduced to wild type embryos (bottom). The “mix” indicates mixture of equal amounts of mRNAs of the three genes. The statistical assays were performed using data obtained at 12 hpf. (F) Time-matched bright field images of embryo showing developmental delay and cytolysis (top) and the rate of developmental abnormality (bottom) when shRNA of nfkbiaa, perp and mgll were introduced to wild type embryos. The “mix” indicates mixture of equal amount of shRNAs of the three genes. Error bars, mean ± s.d., n = 3 (biological replicates).