Fig. 4
- ID
- ZDB-FIG-220811-4
- Publication
- Yan et al., 2022 - Irf2bp2a regulates liver development via stabilizing P53 protein in zebrafish
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Fig. 4. C/ebpα is epistatic to irf2bp2a in liver development. (A) Luciferase reporter assay. Bars showed the relative luciferase activity on the zebrafish irf2bp2a promoter (−3.1 kb) (Student t-test, N ≥ 3. Error bars represent mean ± SD,****P < 0.0001). (B) CHIP-PCR assay in zebrafish larvae expressing GFP or GFP-C/ebpα using an anti-GFP antibody. The irf2bp2a promoter region could be specifically co-immunoprecipitated with GFP-C/ebpα. (Student t-test, N ≥ 3. Error bars represent mean ± SD,****P < 0.0001). (C) WISH assay of lfabp in WT, c/ebpα−/− embryos and c/ebpα−/− embryos injected with irf2bp2a mRNA. (E) WISH assay of lfabp in WT, irf2bp2a−/− embryos and irf2bp2a−/− embryos injected with c/ebpα mRNA. (D, F) The relative liver area measured. The result shown is fold difference compared with the level detected in control embryos (Student t-test, N ≥ 3. Error bars represent mean ± SD, ns, not significant, ****P < 0.0001). (G) Model of irf2bp2a regulatory network in liver development. A C/ebpα-Irf2bp2a-P53 axis controls liver development in zebrafish. As a downstream target of C/ebpα, Irf2bp2a can compete with Mdm2 to recruit Irf2, which in turn affects the stability of P53 protein. |
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| Stage: | Protruding-mouth |
Reprinted from Biochimica et biophysica acta. General subjects, 1866(10), Yan, L., Gao, S., Zhu, J., Zhou, J., Irf2bp2a regulates liver development via stabilizing P53 protein in zebrafish, 130186, Copyright (2022) with permission from Elsevier. Full text @ BBA General Subjects