Fig. 4

Fig. 4

Fig. 4. C/ebpα is epistatic to irf2bp2a in liver development. (A) Luciferase reporter assay. Bars showed the relative luciferase activity on the zebrafish irf2bp2a promoter (−3.1 kb) (Student t-test, N ≥ 3. Error bars represent mean ± SD,****P < 0.0001). (B) CHIP-PCR assay in zebrafish larvae expressing GFP or GFP-C/ebpα using an anti-GFP antibody. The irf2bp2a promoter region could be specifically co-immunoprecipitated with GFP-C/ebpα. (Student t-test, N ≥ 3. Error bars represent mean ± SD,****P < 0.0001). (C) WISH assay of lfabp in WT, c/ebpα^−/− embryos and c/ebpα^−/− embryos injected with irf2bp2a mRNA. (E) WISH assay of lfabp in WT, irf2bp2a^−/− embryos and irf2bp2a^−/− embryos injected with c/ebpα mRNA. (D, F) The relative liver area measured. The result shown is fold difference compared with the level detected in control embryos (Student t-test, N ≥ 3. Error bars represent mean ± SD, ns, not significant, ****P < 0.0001). (G) Model of irf2bp2a regulatory network in liver development. A C/ebpα-Irf2bp2a-P53 axis controls liver development in zebrafish. As a downstream target of C/ebpα, Irf2bp2a can compete with Mdm2 to recruit Irf2, which in turn affects the stability of P53 protein.