Fig. 2

Fig. 2. Foxc1a is expressed in ventricular cardiomyocytes after 64 hpf. A and B: Confocal sagittal sections of foxc1a^nju80 embryos at 19 ss and 26 hpf. The white arrow in (A) points to the cardiac cone region. A lateral view with the abdomen oriented to the left and the head upward (A). A lateral view with the abdomen oriented to the right and the head upward (B). C–I: Confocal sagittal sections of Tg(myl7:nDsRed);foxc1a^nju80/+ in-crossed embryos from 32 hpf to 108 hpf. All images are ventral views with the heads oriented upward. The myl7⁺ cells are labeled in red, and the foxc1a⁺ cells are labeled in green. The white arrows in (F–I) indicate representative colocalized cells. J and K: Representative projections of in vivo confocal images show the representative hearts of Tg(kdrl:ras-mCherry);foxc1a^nju80/+ in-crossed embryos. Both images show ventral views with the head upward. The white arrow in (K) indicates a representative foxc1a⁺ cell. The kdrl⁺ cells are labeled in red, and the foxc1a⁺ cells are labeled in green. Scale bars, 50 μm. ss, somite stage; hpf, postfertilization.