Fmnl2 is required for remodeling of gliovascular interactions. a dIHCs for S100β (astroglia) and ZO-1 (tight junctions/vessels) with DAPI counterstain. a′, a′′ Individual channels for the inset in a. b Higher magnification view of dorsoventral gliovascular (GV) junctions. b′ Close-up of inset in b. Arrows indicate overlaps. c, d dIHCs for kdrl:GFP (blood vessels) and S100β in control (c) and amyloid-injected (d) brains with DAPI counterstain. c′, d′ DAPI omitted from c and d. e Representative snapshot of the automated quantification of the surface of gliovascular interactions. f Quantification results of GV interactions. g, h dIHCs for kdrl:GFP and S100β in amyloid injected brains that were co-injected with control morpholino (g) and fmnl2a/b morpholino (h) with DAPI counterstain. g′, h′ DAPI omitted from g and h. i Close-up image of blood vessels interacting with glial endfeet. j Quantification results of GV interactions. Yellow arrows in c′, d′, g′ and h′ indicate exemplary gliovascular interactions. Scale bars as indicated
|
