ZFIN Image: Lee et al., 2022, Fig. 3

Image

Figure Caption

Fig. 3

Fmnl2 is required for remodeling of gliovascular interactions. a dIHCs for S100β (astroglia) and ZO-1 (tight junctions/vessels) with DAPI counterstain. a′, a′′ Individual channels for the inset in a. b Higher magnification view of dorsoventral gliovascular (GV) junctions. b′ Close-up of inset in b. Arrows indicate overlaps. c, d dIHCs for kdrl:GFP (blood vessels) and S100β in control (c) and amyloid-injected (d) brains with DAPI counterstain. c′, d′ DAPI omitted from c and d. e Representative snapshot of the automated quantification of the surface of gliovascular interactions. f Quantification results of GV interactions. g, h dIHCs for kdrl:GFP and S100β in amyloid injected brains that were co-injected with control morpholino (g) and fmnl2a/b morpholino (h) with DAPI counterstain. g′, h′ DAPI omitted from g and h. i Close-up image of blood vessels interacting with glial endfeet. j Quantification results of GV interactions. Yellow arrows in c′, d′, g′ and h′ indicate exemplary gliovascular interactions. Scale bars as indicated

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Acta Neuropathol.