FIGURE

Fig. 1

ID

ZDB-FIG-220623-27

Publication

Arnold et al., 2022 - mafba and mafbb differentially regulate lymphatic endothelial cell migration in topographically distinct manners

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Fig. 1

Figure 1. mafba and mafbb are required for facial lymphatic development (A and B) Confocal images of (A) face and (B) trunk lymphatics labeled with Tg(fli1a:nEGFP) (endothelial nuclei, green) and Tg(-5.2lyve1b:DsRed2) (VECs and LECs, grey) in sibling and mafbb mutants at 5 dpf. LFL, lateral facial lymphatic; MFL, medial facial lymphatic; LAA, branchial lymphatic arches; OLV, otolithic lymphatic vessel; DLLV, dorsal longitudinal lymphatic vessel; ISLV, intersegmental lymphatic vessel; TD, thoracic duct. Scale bars, 100 μm. (C) Quantification of LECs in the face and trunk based on (A) in wild-type (mafbb+/+), mafbb heterozygous (mafbb+/uq47bh), and mafbb homozygous mutants (mafbbuq47bh) at 5 dpf. Embryos per genotypes; n = 10. One-way ANOVA: ∗p = 0.0174 for mafbb+/uq47bh versus mafbbuq47bh in face LECs, ns p ≥ 0.177 for other comparisons. (D and E) Confocal images of (D) facial and (E) trunk lymphatics at 5 dpf for siblings and mafba and mafba;mafbb mutants. Transgenic markers described in (A). ∗: absent lymphatics. Scale bars, 100 μm. (F) LEC quantification at 5 dpf; embryos per genotype; n = 17 (face), n = 11 (trunk), n = 10 (muLECs). One-way ANOVA: p values for facial LECs: ns p = 0.7733 for siblings versus mafbb mutants; ∗∗∗∗p < 0.0001 for siblings versus mafba or mafba;mafbb mutants and for mafbb versus mafba or mafba;mafbb mutants; ∗∗p < 0.0047 for mafba versus mafba;mafbb mutants. p values for trunk LECs: ∗∗∗p ≤ 0.0004 for siblings versus mafba or mafba;mafbb and mafba versus mafbb mutants; ∗∗p ≤ 0.0029 for mafbb versus mafba;mafbb mutants. Other comparisons: ns p ≥ 0.9999. Kruskal-Wallis test for muLECs: ns p = 0.070. (G) Gross morphology of embryos at 5 dpf. ∗: absent structures; OV, otolithic vesicle; SB, swim bladder. Scale bars, 1 mm. ns, non-significant; stars are significant; error bars are mean ±SD. See also Figure S1.

Expression Data

Genes:	DsRed EGFP
Fish:	nz101Tg; y7Tg mafba^uq4bh/uq4bh; nz101Tg; y7Tg mafbb^{uq47bh/uq47bh}; nz101Tg; y7Tg mafba^uq4bh/uq4bh; mafbb^{uq47bh/uq47bh}; nz101Tg; y7Tg
Anatomical Terms:	dorsal longitudinal lymphatic vessel dorsal longitudinal lymphatic vessel nucleus facial lymphatic network nucleus intersegmental lymph vessel intersegmental lymph vessel nucleus lateral facial lymph vessel medial facial lymph vessel otolithic lymph vessel posterior cardinal vein nucleus thoracic duct thoracic duct nucleus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mafba^uq4bh/uq4bh; nz101Tg; y7Tg mafbb^{uq47bh/uq47bh}; nz101Tg; y7Tg mafba^uq4bh/uq4bh; mafbb^{uq47bh/uq47bh}; nz101Tg; y7Tg
Observed In:	axial lymph vessel facial lymphatic network facial lymphatic network endothelial cell intersegmental lymph vessel intersegmental lymph vessel endothelial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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