FIGURE

Fig. 7

ID

ZDB-FIG-220602-19

Publication

America et al., 2022 - An integrated model for Gpr124 function in Wnt7a/b signaling among vertebrates

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Fig. 7

Figure 7. Functional comparison of mouse and zebrafish Gpr124 extracellular and transmembrane domains (A and D) Schematics of the investigated mouse and zebrafish Gpr124 chimeras. (B and E) Luciferase dose-response assays in HEK293 STF cells co-transfected with Wnt7a, Fz1, Reck, and the indicated Gpr124 chimeras. Data are normalized to ELISA data, n = 3. (C and F) Quantification of hindbrain CtAs in 48 hpf gpr124 morphant embryos injected at the one-cell stage with 100 pg of the indicated mRNAs. (G) Model for the various modalities of Gpr124/Reck-dependent Wnt7a/b signaling and the importance of their relative contribution in different species. Related to Figures S5 and S6.

Expression Data

Gene:	EGFP
Fish:	s843Tg/s843Tg + MO1-adgra2
Knockdown Reagent:	MO1-adgra2
Anatomical Terms:	hindbrain central artery primordial hindbrain channel
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg/s843Tg + MO1-adgra2
Knockdown Reagent:	MO1-adgra2
Observed In:	central artery Wnt receptor activity central artery Wnt-Frizzled-LRP5/6 complex hindbrain central artery primordial hindbrain channel
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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