Figure 3

Lactate regulated the expression of monocarboxylate transporters (MCTs) and epithelial–mesenchymal transition (EMT) markers in glioblastoma cells. (A–C) Protein expressions of MCT1, MCT4, β-catenin, and E-cadherin in U-87 MG cells (A), A-172 cells (B), and U-251 MG cells (C) following 72 h of lactate (20 mM) treatment. The figures presented are representative of at least four independent experiments, and values represent the mean ± SEM of experiments performed in quadruplicate. (D)HCAR1 gene expression in U-87 MG, A-172, and U-251 MG cells following 24 h of lactate (20 mM) treatment. (E–G) Gene expressions of HCAR1(E), MCT1(F), and MCT4(G) in U-87 MG, A172, and U-251 MG cells following 24 h of 3,5-dihydroxybenzoic acid (3,5-DHBA, 150 μM) treatment. Values represent the mean ± SEM of experiments performed in quadruplicate. P-values <0.05 were considered as statistically significant (**p < 0.01; ***p < 0.001 vs. untreated).