FIGURE

Figure 6

ID

ZDB-FIG-220515-6

Publication

Xu et al., 2022 - Knockout of Nur77 Leads to Amino Acid, Lipid, and Glucose Metabolism Disorders in Zebrafish

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Figure 6

Nur77 regulates insulin secretion and β-cell number in zebrafish larvae. (A) Heatmaps of transcripts in insulin signaling pathway. Colors represent high (red), low (blue), or average (white) expression values based on Z-score-normalized fragments per kilobase per million mapped read (FPKM) values for each gene. (B, C) The validation of the expression levels of insulin genes by qRT-PCR analysis in WT and nur77^−/− zebrafish larvae, (B)insa (n = 3), (C) and insb (n = 3). (D, E) The insulin contents in WT and nur77^−/− zebrafish larvae. (D) Representative images of the fluorescence of α cell and β cell from control and nur77^−/−; α cells are indicated by the green fluorescence with Tg(gcg:eGFP), β cell are indicated with red fluorescence by immunostaining with insulin antibody. (E) Quantification of β-cell fluorescence intensity from control and nur77^−/−. The number of larvae (n = 12~18). (F, G) The number of pancreatic β cells in Tg(Ins:H2BmCherry) and nur77^−/−;Tg(Ins:H2BmCherry) zebrafish larvae. (F) Representative images of the β-cell (red) number in Tg(Ins:H2BmCherry) and nur77^−/−;Tg(Ins:H2BmCherry) at 6 dpf. (G) Quantification of β-cell number in Tg(Ins:H2BmCherry) and nur77^−/−;Tg(Ins:H2BmCherry) from 4 to 7 dpf zebrafish larvae (n = 19~50). (H, I) The glucose-stimulated GCaMP6s response in β cells of Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s) and nur77^−/−;Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s). (H) Representative images of GCaMP6s response in β cells of Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s) and nur77^−/−;Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s) by 5 or 20 mM glucose ECS solution; green signal is GCaMP6. (I) Quantification of GCaMP6s response (GFP fluorescence intensity) in β cells of Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s) and nur77^−/− nur77^−/−;Tg(Ins:H2BmCherry);Tg(Ins:GCaMP6s). Results are represented as means with standard errors (n = 13~20); ns, no significance; ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 by t-test.

Expression Data

Gene:	insb
Fish:	nr4a1^{xmu201/xmu201} (AB)
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nr4a1^{xmu201/xmu201} (AB) nr4a1^{xmu201/xmu201}; tud202Tg; vu513Tg nr4a1^{xmu201/xmu201}; vu513Tg
Observed In:	insulin secretion involved in cellular response to glucose stimulus pancreatic B cell whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)