FIGURE

Fig 7

ID

ZDB-FIG-220426-33

Publication

Gibson et al., 2022 - Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection

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Fig 7

Cryptococcal infection leads to increased tension across VE-cadherin.

A-C Infection of FRET tension reporter (VE-cadherin-TS) transgenic zebrafish line with 1000 cfu mCherry C. neoformans A-B FRET = YFP signal via FRET with excitation of CFP, CFP = CFP signal with direct excitation of CFP, YFP = YFP signal with direct excitation of YFP. A images of non-infected control vessels B Image showing infected fish, vessel containing a mass (left) and a vessel without a mass (right) C FRET analysis of infected fish with or without masses and non-infected controls larvae (n = 2, 4–7 larvae per repeat, +/- SD, **p<0.01, Kruskal-Wallis test, where vessel fluorescence was measured at each side of vessel) D Proportion of infected fish with disseminated infection. 7 repeats, 10 zebrafish larvae per repeat per group. P = 0.036 unpaired t-test.

Expression Data

Genes:	mCherry YFP
Fish:	uq11bhTg/uq11bhTg
Conditions:	biological treatment: Cryptococcus neoformans, chemical treatment by environment: GI254023X biological treatment: Cryptococcus neoformans
Anatomical Term:	trunk intersegmental vessel
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uq11bhTg/uq11bhTg
Conditions:	biological treatment: Cryptococcus neoformans, chemical treatment by environment: GI254023X biological treatment: Cryptococcus neoformans
Observed In:	intersegmental vessel intersegmental vessel symbiont entry into host
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

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