FIGURE

Figure 3

ID

ZDB-FIG-220411-3

Publication

Sheng et al., 2022 - MicroRNA-22 coordinates vascular and motor neuronal pathfinding via sema4 during zebrafish development

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Deficiency of mir-22a caused aberrant axonal projection of PMNs. (a) Confocal imaging analysis of primary motor neurons in the control embryos, miR-22a morphants and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos at 48 and 72 hpf. Arrowheads indicate the CaPs across different segments. (b,c) Schematic diagram for primary motor neurons in the control embryos and miR-22a morphants. (d) Statistical analysis of the ratio of CaPs across different segments in the control (n = 8), miR-22a morphants (n = 8) and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos (n = 8) at 48 and 72 hpf; One-way ANOVA, ****p < 0.0001. (e) Statistical analysis of the ratio of aberrant axonal projection of CaPs in the control (n = 8), miR-22a morphants (n = 8) and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos (n = 8) at 48 and 72 hpf; one-way ANOVA, ****p < 0.0001.

Expression Data

Gene:	GFP
Fish:	ml2Tg ml2Tg + MO1-dre-mir-22a
Knockdown Reagent:	MO1-dre-mir-22a
Anatomical Terms:	CaP motoneuron axon MiP motor neuron axon RoP motor neuron axon
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg + MO1-dre-mir-22a
Knockdown Reagent:	MO1-dre-mir-22a
Observed In:	CaP motoneuron axon extension involved in axon guidance
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.