FIGURE

Figure 1

ID

ZDB-FIG-220411-1

Publication

Sheng et al., 2022 - MicroRNA-22 coordinates vascular and motor neuronal pathfinding via sema4 during zebrafish development

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Figure 1

Deficiency of miR-22a caused aberrant vascular networks. (a) Confocal imaging analysis of control MO, miR-22a-MO and miR-22a-sponge-injected Tg(kdrl:EGFP) embryos at 30 hpf and 48 hpf. The arrowheads indicate the aberrant angiogenic sprouts. A: dorsal aorta; V: posterior cardinal vein. (b) Statistics of horizontal sprouts ratio in 30 hpf embryos injected with control MO (n = 7), miR-22-MO (n = 7) and miR-22-sponge (n = 7). One-way ANOVA, **** p < 0.0001. (c) Statistics of embryos with vessel across segments ratio in each group: control MO (n = 6), miR-22a-MO (n = 8) and miR-22-sponge (n = 8). One-way ANOVA, ** p < 0.01; **** p < 0.0001. (d) Confocal imaging analysis of control and miR-22a-KO Tg(fli1a:EGFP-CAAX) embryos at 48 hpf. (e) The statistics of embryos with vessels across segments ratio in each group: control (n = 7) and miR-22-KO (n = 7). t-test, ****, p < 0.0001.

Expression Data

Gene:	EGFP
Fish:	s843Tg y1Tg s843Tg + MO1-dre-mir-22a y1Tg + TALEN1-dre-mir-22a + TALEN2-dre-mir-22a + TALEN3-dre-mir-22a + TALEN4-dre-mir-22a
Knockdown Reagents:	MO1-dre-mir-22a TALEN1-dre-mir-22a TALEN2-dre-mir-22a TALEN3-dre-mir-22a TALEN4-dre-mir-22a
Anatomical Terms:	dorsal aorta blood vessel endothelial cell intersegmental vessel blood vessel endothelial cell posterior cardinal vein blood vessel endothelial cell
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg + MO1-dre-mir-22a y1Tg + TALEN1-dre-mir-22a + TALEN2-dre-mir-22a + TALEN3-dre-mir-22a + TALEN4-dre-mir-22a
Knockdown Reagents:	MO1-dre-mir-22a TALEN1-dre-mir-22a TALEN2-dre-mir-22a TALEN3-dre-mir-22a TALEN4-dre-mir-22a
Observed In:	intersegmental vessel intersegmental vessel angiogenesis
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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