The eye structure, feeding behavior and gene expression differences between the wild-type and bmp7b-/- zebrafish. (A) Whole-mount in situ hybridization of the bmp7b at 24 hpf. The blue area represents the zebrafish eyes. (B) The external physical characteristics of eyes. (C) Comparative analysis of the proportion of iris diameter in eye diameter in different points (30, 60, and 90 dpf). (D) HE staining results of eye tissue sections. 1, cornea; 2, retina; 3, sclera; 4, choroid; 5, iris; 6, lens; 7, pigment cell epithelium and photosensitive layer; 8, outer granular layer; 9, outer reticulum layer; 10, inner granule Layer; 11, inner reticulum layer; 12, pigment cell layer; 13, rod cell layer; 14, cone cell layer. (E) Thickness measurement of melanin layer in different regions of eye tissue slices (n = 6). (F) Heat map showing trajectories of adults within 5 s after stimulation (adding brine shrimp) (six repetitions). The red triangle area represents the location of adding brine shrimp. (G) Reaction time and movement distance of adult zebrafish within 5 s after adding brine shrimp. (H) Angular velocities of juvenile zebrafish within 5 s after the light intensity was reduced (from light to dark). Statistically significant differences are marked as *p < 0.05 and **p < 0.01.
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