Behavioral phenotype of mao mutants. (A) mao?/? larvae were hypoactive during diurnal and nocturnal (gray shaded area) locomotor activity tracking at 6 dpf. n=16 for mao+/+, n=16 for mao+/? and n=15 for mao?/?. (B) Larvae at 6 dpf were exposed to alternating 2 min periods of light and darkness (gray shaded areas), with three periods of darkness in total. n=15 mao+/+ and n=16 for mao+/? and mao?/?. (C-E) Analysis of the larval response to sudden darkness [first (C), second (D) and third (E) flash response] in 1 s bins. Gray shaded areas indicate periods of darkness. (F) Mean values for maximum velocity during ten acoustic/vibrational stimuli with a 20 s interstimulus interval (ISI). n=15 for mao+/+, n=16 for mao+/? and n=12 for mao?/?. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. #P<0.05 (mao+/? versus mao+/+ and mao?/?), *P<0.05 (mao+/+ and mao+/? versus mao?/?), §P<0.05 (mao+/+ versus mao?/?), ?P<0.05 (mao+/? versus mao?/?), ?P<0.05 (mao+/? versus mao+/+).
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