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Fig. 1. Behavioral phenotype of mao mutants. (A) mao−/− larvae were hypoactive during diurnal and nocturnal (gray shaded area) locomotor activity tracking at 6 dpf. n=16 for mao+/+, n=16 for mao+/− and n=15 for mao−/−. (B) Larvae at 6 dpf were exposed to alternating 2 min periods of light and darkness (gray shaded areas), with three periods of darkness in total. n=15 mao+/+ and n=16 for mao+/− and mao−/−. (C-E) Analysis of the larval response to sudden darkness [first (C), second (D) and third (E) flash response] in 1 s bins. Gray shaded areas indicate periods of darkness. (F) Mean values for maximum velocity during ten acoustic/vibrational stimuli with a 20 s interstimulus interval (ISI). n=15 for mao+/+, n=16 for mao+/− and n=12 for mao−/−. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. #P<0.05 (mao+/− versus mao+/+ and mao−/−), *P<0.05 (mao+/+ and mao+/− versus mao−/−), §P<0.05 (mao+/+ versus mao−/−), €P<0.05 (mao+/− versus mao−/−), ‡P<0.05 (mao+/− versus mao+/+).