FIGURE
Fig. 7.
- ID
- ZDB-FIG-220304-8
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- Allanki et al., 2021 - Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration
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Fig. 7.
Il-11 signaling in endothelial cells allows CM repopulation after cardiac injury. (A) Experimental design and confocal images of immunostaining (Aldh1a2, magenta; αSMA, yellow) on cryosections from vehicle-treated il11ra+/− siblings (n = 6) and vehicle- or 4-OHT–treated il11ra−/− (n = 4 and 5, respectively) Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (B and C) Quantification of total αSMA+ cell density (B) and αSMA+ Aldh1a2+ cell density (C) in the injured area, 7 dpci. (D) Experimental design and F-actin staining on 50-μm-thick cryosections from vehicle-treated il11ra+/− siblings and vehicle- or 4-OHT–treated il11ra−/− Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (E and F) Quantification of the number per ventricle (E) (il11ra+/− veh, n = 6; il11ra−/− veh, n = 4; il11ra−/− 4-OHT, n = 5) and length (F) (il11ra+/− veh, n = 481; il11ra−/− veh, n = 185; il11ra−/− 4-OHT, n = 437) of CM protrusions at 7 dpci. Data represent means ± SD (B, C, E, and F). One-way ANOVA (B, C, and E); Kruskal-Wallis test (F). n, ventricles (A and E); n, CM protrusions (F). Yellow dashed lines demarcate the injured area (A and D); white arrowheads point to αSMA+ Aldh1a2+ endocardial cells (A, insets). Scale bars, 50 μm (A and D) and 10 μm (A, insets).
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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