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Fig. 7.

ID: ZDB-IMAGE-220304-8
Source: Figures for Allanki et al., 2021

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Figure Caption

Fig. 7. Il-11 signaling in endothelial cells allows CM repopulation after cardiac injury.

(A) Experimental design and confocal images of immunostaining (Aldh1a2, magenta; αSMA, yellow) on cryosections from vehicle-treated il11ra^+/− siblings (n = 6) and vehicle- or 4-OHT–treated il11ra^−/− (n = 4 and 5, respectively) Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (B and C) Quantification of total αSMA⁺ cell density (B) and αSMA⁺ Aldh1a2⁺ cell density (C) in the injured area, 7 dpci. (D) Experimental design and F-actin staining on 50-μm-thick cryosections from vehicle-treated il11ra^+/− siblings and vehicle- or 4-OHT–treated il11ra^−/− Tg(fli1:CreER); Tg(hsp70l:LBL-il11ra-p2a-mCh) ventricles at 7 dpci. (E and F) Quantification of the number per ventricle (E) (il11ra^+/− veh, n = 6; il11ra^−/− veh, n = 4; il11ra^−/− 4-OHT, n = 5) and length (F) (il11ra^+/− veh, n = 481; il11ra^−/− veh, n = 185; il11ra^−/− 4-OHT, n = 437) of CM protrusions at 7 dpci. Data represent means ± SD (B, C, E, and F). One-way ANOVA (B, C, and E); Kruskal-Wallis test (F). n, ventricles (A and E); n, CM protrusions (F). Yellow dashed lines demarcate the injured area (A and D); white arrowheads point to αSMA⁺ Aldh1a2⁺ endocardial cells (A, insets). Scale bars, 50 μm (A and D) and 10 μm (A, insets).

Acknowledgments

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