FIGURE

Fig. 4

ID

ZDB-FIG-220127-6

Publication

Nord et al., 2021 - Genetic compensation between Pax3 and Pax7 in zebrafish appendicular muscle formation

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Fig. 4

Adult pax3a?/?/3b?/?/7a?/? mutants have restored pectoral fin muscle. Ventral view of adult 12?months old, A, wt and, B, pax3a?/?/3b?/?/7a?/? mutants (n = 3) showing pectoral fin muscle. Dashed black line indicates area of cross section shown in C,D. Phalloidin staining of pectoral fin sections of 12?months old, C, wt and, D, pax3a?/?/3b?/?/7a?/? mutants. BrdU (red) incorporation between 72 and 96 hpf in pectoral fins of, E, wt (n = 51) and, F, pax3a?/?/3b?/?/7a?/? mutant (n = 7), dashed line indicates level of confocal cross sections shown in E' and F' where F-actin is visualized in green. Ventral view of 9 dpf juveniles stained with F310 antibody (green) in G wt (n = 49) and, H, pax3a?/?/3b?/?/7a?/? mutant (n = 4). Missing or reduced muscle is indicated by *. abd, abductor; add, adductor; sh, sternohyoideus. A,B, Scale bar: 1?mm, CD,G,H, 200??m, E,F, 50??m

Expression Data

Antibody:	Ab-F310
Fish:	WT pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5
Anatomical Terms:	primitive pectoral fin abductor primitive pectoral fin adductor sternohyoid
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5
Observed In:	pectoral fin musculature pectoral fin musculature apoptotic process pectoral fin musculature cell population proliferation primitive pectoral fin abductor skeletal muscle cell sternohyoid
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

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