FIGURE

Fig. 6

ID

ZDB-FIG-220124-28

Publication

Taler et al., 2019 - Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate

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Fig. 6

Cellular masses are disorganized and partially differentiated towards lens fiber fate. (A–H) Single confocal planes of eyes from normal (A,A’,C,E,G) and plod3^vu222/vu222 (B,B’,D,F,H) embryos labeled (red) for ZO-1 (A,A’,B,B’), Pax6 (C,D), Prox1 (E,F) and Aq0 (G,H). Nuclei (DAPI) are blue. A’ and B’ are higher magnifications of the regions marked by rectangles in A and B, respectively. Areas of cellular masses are encircled by white lines in D,F,H. Arrows in A point at the ALE and in C at Prox1-positive differentiating lens fiber cells. Insets in C and D show only the Pax6 channel in the region of the ALE and cell mass, respectively. Ages of embryos are demarcated on panels. Number of eyes imaged from two repetitions of the experiments are: A = 9, B = 14, C = 7, D = 11, E = 6, F = 19, G = 15, H = 17. L, lens. Scale bars are 20 μm.

Expression Data

Antibodies:	Ab1-mip Ab1-pax6 Ab1-tjp1 Ab13-prox1
Fish:	WT plod3^vu222/vu222
Anatomical Terms:	lens epithelium lens epithelium tight junction
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plod3^vu222/vu222
Observed In:	lens epithelium lens epithelium lens fiber cell differentiation lens epithelium tight junction
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 458(2), Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A., Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate, 177-188, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.