FIGURE

Fig. 3

ID

ZDB-FIG-211126-9

Publication

Jamadagni et al., 2021 - Chromatin remodeller CHD7 is required for GABAergic neuron development by promoting PAQR3 expression

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Fig. 3

Transcriptomic analysis was performed on dissected 5 dpf brains. A. Volcano plot showing each gene plotted according to its log2 fold change. All highly differentially expressed genes with P < 0.05 are in orange with fold change > 1.5. B, C. Biological processes (B) and molecular function (C) that are enriched in the differentially expressed genes. D. ERK activation by phosphorylation is increased in mutants as shown by Western blot quantification (N = 4; ***P < 0.005; Student’s t-test). E. pERK immunohistochemistry showing increased ERK activation in the mutant brain (N = 3, n = 8). F, G. Treatment with the ERK signalling inhibitor U0126 ameliorated the number of GABAergic neuron (n = 10–15; **P < 0.05; ****P < 0.0001, one-way ANOVA). H. Treatment with the ERK signalling inhibitor U0126 rescues locomotor hyperactivity phenotype in chd7 mutant fish (n = 8 for chd7+/+ and chd7−/−; n = 16 for U0126-treated fish; ****P < 0.0001; ns, not significant, one-way ANOVA). Data information: Data are presented as mean ± SEM. Scale bar = 50 μm. n represents number of fish used. N represents number of experimental repeats.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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