FIGURE

Fig. 4

ID

ZDB-FIG-211012-4

Publication

Rattka et al., 2021 - Spen deficiency interferes with Connexin 43 expression and leads to heart failure in zebrafish

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Fig. 4

(A) By screening for dysregulated genes via a cardiovascular disease assay we identified gap junction protein Connexin 43 (Cx43) to be downregulated (supplementary material Fig. A.3A) in Spen-deficient embryos 72 hpf, which was confirmed by cx43-specific qPCR in Spen-deficient hearts (0.47 ± 0.10, p = 0.0063, n = 3) as well as Spen-deficient embryos (0.46 ± 0.04, p = 0.0001, n = 3). (B) Western Blot analysis verifies downregulation of Cx43 in Spen-deficient embryos on the protein level. (C) Quantification of Cx43 levels in Western Blots of Spen-deficient and control embryos (n = 3). Images (B) have been cropped. A, C: Assessment of significance by t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

Expression Data

Gene:	gja1b
Fish:	AB/TU + MO2-spen
Knockdown Reagent:	MO2-spen
Anatomical Term:	heart
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO2-spen
Knockdown Reagent:	MO2-spen
Observed In:	heart
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Journal of Molecular and Cellular Cardiology, 155, Rattka, M., Westphal, S., Gahr, B.M., Just, S., Rottbauer, W., Spen deficiency interferes with Connexin 43 expression and leads to heart failure in zebrafish, 25-35, Copyright (2021) with permission from Elsevier. Full text @ J. Mol. Cell. Cardiol.