FIGURE

Fig 2

ID

ZDB-FIG-210912-2

Publication

Minenkova et al., 2021 - Is impaired energy production a novel insight into the pathogenesis of pyridoxine-dependent epilepsy due to biallelic variants in ALDH7A1?

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Fig 2

Decreased levels of vitamin B6 vitamers in aldh7a1 knock-out 11 dpf embryos relative to age-matched wildtype control embryos.

Vitamin B6 vitamers levels are reduced in aldh7a1 homozygous knock-out embryos relative to the wildtype. It is shown in a scatterplot of quantification of the levels of vitamin B6 vitamers in aldh7a1 homozygous knock-out zebrafish relative to the wildtype as measured by LC-MS. The graph demonstrates decreased levels of vitamin B6 vitamers in aldh7a1 knock-out 11 dpf embryos (aldh7a1 knock-out, grey squares) relative to the age-matched wildtype control embryos (Wildtype, black circles). Each dot represents metabolite data collected from 20 embryos. For a single data point of homozygous aldh7a1 knock-out zebrafish, only 16 embryos were available, so that data point was excluded from the analysis. nd, not detectable–the levels of metabolites could not be measured. p values were calculated by unpaired student t-test for multiple measurements followed by Holm-Sadak correction for multiple analysis. Error bars, SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	aldh7a1^{zf2074/zf2074}
Observed In:	whole organism pyridoxal whole organism pyridoxal 5'-phosphate whole organism pyridoxamine 5'-phosphate whole organism pyridoxine
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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