Figure 2

Synergistic effect of kras^V12 expression and LPS treatment in intestinal tumorigenesis. Four-month-postfertilization WT and kras+ zebrafish were cotreated with 2 μM of mifepristone and 40 ng/mL of LPS for 4 weeks, and samples were then collected for gross observations and histological analyses. There were four experimental groups: WT, WT/LPS, kras+, and kras+/LPS. (A,B) Body length and body weight. (C) Survival curves. (D) Examples of normal intestines, enteritis, hyperplasia, and tubular adenoma as revealed by H&E-stained sections of the intestine. (E) Summary of intestinal histological abnormalities observed in the four experimental groups. These data were generated from results of a blinded histological analysis (WT, N = 20; WT/LPS, N = 20; kras+, N = 20; kras+/LPS, N = 20). Differences among the variables were assessed using Student’s t-tests or one-way ANOVA. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 50 μm.