FIGURE

Figure 1

ID

ZDB-FIG-210902-60

Publication

Naef et al., 2021 - Efficient Neuroprotective Rescue of Sacsin-Related Disease Phenotypes in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Generation of sacs-null mutant zebrafish. (A) Sequencing chromatographs of control and sacs-null mutant (sacs^?/?)specimens and alleles. The red rectangle define frameshift mutation in exon 7. The arrow indicates the area of the induced deletion. (B) qRT-PCR analysis revealed a decrease in the level of sacs mRNA expression, normalized to ?-actin. Three independent RNA samples from sacs^?/? mutant larvae at 120 hpf and from controls were analyzed. * p ? 0.05, calculated by Student?s t-test. (C) Lateral view photographs of representative control and sacs^?/? specimens. (D) No dysmorphology and full length was noted at 120 hpf, but (E) homozygous larvae showed slight but significant ?microphthalmia?, **** p ? 0.00001, calculated by Mann-Whitney test. The values are expressed as mean � standard deviation (SD). Abbreviations: n, total number of evaluated embryos; error bars indicate standard errors of the means; ns, not significant.

Expression Data

Gene:	sacs
Fish:	WT sacs^irc1/irc1
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sacs^irc1/irc1
Observed In:	eye whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.