ZFIN Image: Naef et al., 2021, Figure 1

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Figure 1

Generation of sacs-null mutant zebrafish. (A) Sequencing chromatographs of control and sacs-null mutant (sacs^−/−)specimens and alleles. The red rectangle define frameshift mutation in exon 7. The arrow indicates the area of the induced deletion. (B) qRT-PCR analysis revealed a decrease in the level of sacs mRNA expression, normalized to β-actin. Three independent RNA samples from sacs^−/− mutant larvae at 120 hpf and from controls were analyzed. * p ≤ 0.05, calculated by Student’s t-test. (C) Lateral view photographs of representative control and sacs^−/− specimens. (D) No dysmorphology and full length was noted at 120 hpf, but (E) homozygous larvae showed slight but significant “microphthalmia”, **** p ≤ 0.00001, calculated by Mann-Whitney test. The values are expressed as mean ± standard deviation (SD). Abbreviations: n, total number of evaluated embryos; error bars indicate standard errors of the means; ns, not significant.

Acknowledgments

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