FIGURE

Fig 3

ID

ZDB-FIG-210820-20

Publication

Gao et al., 2021 - Irf2bp2a regulates terminal granulopoiesis through proteasomal degradation of Gfi1aa in zebrafish

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Fig 3

Polymerization and potential ubiquitin E3 ligase function are indispensable for Irf2bp2a in regulating the differentiation of neutrophilss.

(A) Structure, missing function and rescue effect of wild type and variant forms of Irf2bp2a. (B-J) Irf2bp2a mRNA rescue assays in irf2bp2a^-/- embryos. Mpx probe was used in WISH to examine rescue effect with wild irf2bp2a (D), ZM (E), Δzinc finger (F), tet-ZM (G), RM (H), Δring finger (I) and K486R mutant (J) mRNA injections. (K) The statistical significance was calculated by using one-way ANOVA. The statistical significance was calculated using 1-way ANOVA followed by Dunnett T3 correction. The asterisk indicates a statistical difference (N = 5, 15–25 embryos were used for each experiment. Each dot represents the mean value of one experiment. Error bars represent mean ± SEM. ns: no statistical significance; ***P < 0.001).

Expression Data

Gene:	mpx
Fish:	irf2bp2a^{zf3610/zf3610}
Anatomical Term:	neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	irf2bp2a^{zf3610/zf3610}
Observed In:	neutrophil neutrophil regulation of ubiquitin protein ligase activity
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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