FIGURE

Fig 1

ID

ZDB-FIG-210820-18

Publication

Gao et al., 2021 - Irf2bp2a regulates terminal granulopoiesis through proteasomal degradation of Gfi1aa in zebrafish

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Fig 1

The establishment of a zebrafish <italic toggle='yes'>irf2bp2a</italic> knockout line.

(A) Schematic representation of Cas9 target site in the first exon of zebrafish irf2bp2a. The deleted nucleotides in the mutant gene are marked by hyphens. (B) Schematic representation of wild type (493 amino acids) and mutant Irf2bp2a proteins (122 amino acids). The site where the frameshift was introduced is marked by triangles. (C) Western blot analysis of FLAG-tagged wild type and mutant Irf2bp2a proteins expressed in HEK293 cells. (D) Immunofluorescence analysis of FLAG-tagged wild type (top panel) and mutant Irf2bp2a (bottom panel) proteins, demonstrating that the truncated protein lost its nuclear localization. Scale bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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