FIGURE

Fig. 4.

ID

ZDB-FIG-210801-85

Publication

Santra et al., 2021 - Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts

Other Figures

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Fig. 4.

V-ATPase loss reduces the number of hair cells in neuromasts. (A) Acetylated tubulin staining detects hair cells in wild-type, atp6v1f^−/− and atp6v1h^−/− neuromasts at 2 dpf and 4 dpf. Arrowheads point to individual hair cells. (B,C) The number of hair cells per neuromast at 2 dpf and 4 dpf in atp6v1f^−/− embryos and wild-type siblings (B), and atp6v1h^−/− and wild-type siblings (C). (D) Sox2 staining labels neuromast support cells at 2 dpf and 4 dpf. Dashed line circles indicate the neuromast boundary. (E,F) The number of support cells per neuromast at 2 dpf and 4 dpf in atp6v1f^−/− embryos and wild-type siblings (E), and atp6v1h^−/− and wild-type siblings (F). n=number of embryos. ***P=0.0009 and ****P<0.0001 by unpaired Student's t-test with Welch's correction. ns, not significant.

Expression Data

Antibodies:	Ab1-tuba Ab3-sox2
Fish:	WT atp6v1f^{hi1988Tg/hi1988Tg} atp6v1h^{hi923Tg/hi923Tg}
Anatomical Terms:	anterior lateral line neuromast neuromast hair cell anterior lateral line neuromast neuromast support cell neuromast hair cell kinocilium
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp6v1f^{hi1988Tg/hi1988Tg} atp6v1h^{hi923Tg/hi923Tg}
Observed In:	anterior lateral line neuromast neuromast neuromast hair cell neuromast support cell
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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