FIGURE

Fig 5

ID

ZDB-FIG-210801-44

Publication

Vong et al., 2021 - The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish

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Fig 5

A. WISH to detect ddx4 positive PGCs and shh in the midline at Bud stage. PGCs are reduced (scatter plot, right) and ectopically located in MZigf2bp3la659Tg embryos compared to wild type (WT) embryos. B. Migration tracks of PGCs in bud stage embryos, labelled with GFP-nos1 reporter. MZigf2bp3la659Tg embryos show aberrant PGC migration compared to WT controls. C. PGC track analysis showing displacement, speed and straightness index. Ectopic PGCs in MZigf2bp3la659Tg mutants show significantly reduced total displacement and straightness, although speed of individual PGCs is not altered in mutant embryos compared to WT controls. Scale bars, 200 μm; p*<0.05, **<0.01, ***<0.001; Number of PGCs analysed = 31, 16 and 17 for WT, actively migrating and ectopic MZigf2bp3la659Tg PGCs, respectively.

Expression Data

Genes:	ddx4 shha
Fish:	WT igf2bp3^{la020659Tg/la020659Tg}
Anatomical Terms:	axis primordial germ cell
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	igf2bp3^{la020659Tg/la020659Tg}
Observed In:	primordial germ cell
Stage:	Bud

Phenotype Detail

Acknowledgments

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