Loss of rgs4 function alters Akt signaling in neural cells. (A,B) Immunoblotting of lysates from zebrafish embryos at 20 hpf. Akt and p-Akt amounts were normalized to α-tubulin and the ratio of p-Akt relative to Akt was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. Erk and p-Erk amounts were normalized to α-tubulin and the ratio of p-Erk relative to Erk was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. (C) Immunoblotting of lysates from zebrafish embryos at 48 hpf. Akt and p-Akt amounts were normalized to β-actin and the ratio of p-Akt relative to Akt was compared between WT and MZrgs4 lysates. MZrgs4 embryos showed a significant decrease in the amount of p-Akt/Akt in comparison to WT. (D) Immunoblotting of lysates from zebrafish embryos at 48 hpf. Erk and p-Erk amounts were normalized to Gapdh and the ratio of p-Erk relative to Erk was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. (E) Immunoblotting of neural lysates (or extracts) from zebrafish embryos at 48 hpf. Akt and p-Akt amounts were normalized to β-actin and the ratio of p-Akt relative to Akt was compared between WT and MZrgs4 lysates. MZrgs4 embryos showed a significant decrease in the amount of p-Akt/Akt in comparison to WT.
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