FIGURE

Figure 2

ID

ZDB-FIG-210708-24

Publication

Mikdache et al., 2021 - Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish

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Figure 2

MZrgs4 embryos show motility and motoneurons axon outgrowth defects. (A) Quantification of the percentage of embryos that showed a normal response to the touch test at 48 hpf in WT (average of 94.44 ± 5.556, n = 18) and MZrgs4 embryos (average of 30.77 ± 7.48, n = 39). (B,C) Z projections of confocal microscopy images of HuC:gfp (B) and HuC:gfp/MZrgs4 mutant (C) showing the extending axons of primary motoneurons at 22 hpf. Arrows indicate the tip ends of extending axons. Scale bar = 20 μm. (D) Quantification of the motor axons length at 22 hpf in HuC:gfp (average of 30.20 ± 1.78 μm, 25 motor axons, n = 9) and MZrgs4/HuC:gfp (average of 31.14 ± 1.01 μm, 36 motor axons, n = 12). (E–H) Z Projections of whole-mount immunostaining for Znp1 showing the extending axons of primary motoneurons at 30 hpf in WT (E) and MZrgs4 (F) and the ramifications or branching of these motoneurons at 48 hpf in WT (G) and MZrgs4 embryos (H) Scale bar = 25 μm. (I) Quantification of the motor axons length at 30 hpf in WT (average of 106.7 ± 2.44 μm, 42 motor axons, n = 12) and MZrgs4 embryos (average of 64.37 ± 6.66 μm, 50 motor axons, n = 12). (J) Quantification of the number of ramifications per motoneuron at 48 hpf in WT (average of 20.64 ± 1.10, 36 motoneurons, n = 11) and MZrgs4 embryos (average of 9.37 ± 0.86, 53 motoneurons, n = 16). (K) Acetylated tubulin expression in WT and MZrgs4 embryo at 48 hpf showing the PLLn nerve (white arrows). Scale bar = 50 μm.

Expression Data

Gene:	EGFP
Antibodies:	Ab1-tuba znp-1
Fish:	WT rgs4^kbc2/kbc2 knu3Tg rgs4^kbc2/kbc2; knu3Tg
Anatomical Terms:	CaP motoneuron axon MiP motor neuron axon motor neuron axon motor neuron cell body posterior lateral line nerve axon RoP motor neuron axon
Stage Range:	26+ somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgs4^kbc2/kbc2 rgs4^kbc2/kbc2; knu3Tg
Observed In:	CaP motoneuron axon CaP motoneuron axon extension posterior lateral line nerve posterior lateral line nerve axon extension primary motor neuron axon primary motor neuron axon extension thigmotaxis
Stage Range:	26+ somites to Long-pec

Phenotype Detail

Acknowledgments

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