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Chimeric analyses demonstrate that cranial neural crest require <italic toggle='yes'>gata3</italic>.(A-C) Wild type sox10:mCherry CNCC (in red) were transplanted unilaterally into gata3;fli1:EGFP mutant hosts (in green). The resulting embryo was imaged at 30 hpf showing the major contribution to the maxillary is wild type (arrow). (D) Flat mount of 4dpf neurocrania of the same fish showing the rescue of the trabeculae (arrow) compared to the loss of trabeculae on the non-transplanted side of the embryo. (E) Graph depicting the overall trabeculae score of the 10 chimeric embryos with the transplanted sides phenotype significantly improved relative to the non-transplanted sides (Mann-Whitney test P = 0.0052). (F) Wild type ubi:Switch (in green) were transplanted unilaterally into gata3 LOF;sox10:mCherry. Chimeric embryos (n = 4) were imaged at 30 hpf to demonstrate donor cells populating the majority of the maxillary domain. (G) Flat mount of same fish at 4 dpf showing that the green donor cells populate the trabeculae on the transplanted side. (H) Stained flat mount of same embryo at 4 dpf showing complete rescue of the trabeculae phenotype. Compare the transplanted trabeculae to the disorganized and improperly fused trabeculae on the control side.
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