FIGURE

FIGURE 4

ID

ZDB-FIG-210526-18

Publication

Grivas et al., 2021 - Midkine-a Regulates the Formation of a Fibrotic Scar During Zebrafish Heart Regeneration

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 4

Transcriptional profiling of regenerating mdka^cn105 hearts. (A) Total number of differentially expressed genes identified by RNA-seq. Numbers indicate upregulated genes (red) and downregulated genes (blue). (B,C) Circular plots showing representative differentially expressed genes (left semicircle perimeter) and IPA biofunctions and upstream regulators (right semicircle perimeter). Activation z-score scale: green, repression; magenta, activation; white, unchanged. LogFC scale: red, upregulated; blue, downregulated; white, unchanged. (D) qPCR analysis of mdka^+/+ and mdka^cn105 7 dpci hearts. t-test; *P < 0.05, **P < 0.01, ***P < 0.001; Mean ± SD. (E,F) Immunofluorescence staining of GFP and BrdU in 7 dpci Tg(fli1a:GFP) heart sections. (E′,F′) Higher magnification of the dashed lines in (A,F). Arrowheads indicate proliferating endothelial cells. Scale bar (E,F) 100 μm; (E′,F′) 10 μm. (G) Quantification of BrdU⁺/GFP⁺ endothelial cells. n_WT = 13, n_KO = 15; t-test; **P < 0.01; Mean ± SD.

Expression Data

Genes:	col1a1a col1a1b col1a2 col5a1 col5a2a cthrc1a fn1a fn1b hif1aa pcolce2b plod2 postna postnb tgfb3
Fish:	AB mdka^cn105/cn105
Condition:	cryoablation: cardiac ventricle
Anatomical Term:	heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mdka^cn105/cn105 mdka^cn105/cn105; y1Tg
Condition:	cryoablation: cardiac ventricle
Observed In:	endothelial cell proliferation heart
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol