FIGURE

Fig. 5

ID

ZDB-FIG-210520-5

Publication

Bertho et al., 2021 - Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition

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Fig. 5

Single confocal plane of representative dazl^?7/+ or dazl^?7/?7 day 8 gonads and dazl^ae57/+, dazl^?7/+ or dazl^?7/ae57 at 10?days labeled with Vasa (green), LaminB1 (magenta; nuclear envelope,) and DAPI (gray; DNA). (A-A?) Vasa+ GC of dazl^?7/+ (n=2) at day 8, with a folded raisin-like nuclear envelope. (B-B?) Vasa+ GC of dazl^?7/?7 (n=3) at day 8, with an unfolded smooth nuclear envelope of Vasa+ GC of dazlae^57/+ (C-C?, n=2) at 10?days. (D-D?) The change in nuclear appearance corresponds to amplification during cyst formation and is intact in dazlae^57/?7. (E-E?) After the transition at 10?days, the nuclei of Vasa+ GC of dazl^?7/+ remain smooth (n=2). The postmitotic GC nuclei also have a smooth nuclear membrane and a large nucleolus. (F-F?) Vasa+ GC of dazlae^57/?7 (n=5) at 10?days. Mutant GCs are individuals with a folded nuclear membrane rather than a smooth morphology at 10?days. Yellow dotted line delineates the gonad. Blue arrows indicate each note for the corresponding panel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dazl^mu12/mu12 dazl^ae57/+; dazl^mu12/+
Observed In:	germ line cell nuclear envelope
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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