FIGURE

Fig 1

ID

ZDB-FIG-210508-1

Publication

Keatinge et al., 2021 - CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury

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Fig 1

<italic>In vivo</italic> pre-screening identifies highly active sCrRNAs.

A: Example gels to assess sCrRNA in vivo mutation rate by resistance to restriction enzyme digest (RFLP) are shown. These indicate > 90% mutation rate (top), medium mutation rate (middle) and no detectable mutation rate (bottom). Each lane is derived from one animal. The chart shows the distribution of > 350 sCrRNAs by in vivo mutation rate. B-D: Activities of individual sCrRNAs show weak correlation with predicted in silico efficiency using different prediction rules (see results). E: sCrRNA activities are not correlated with their relative position to the start codon in vivo.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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