FIGURE

Figure 4

ID

ZDB-FIG-210503-16

Publication

Kamel et al., 2021 - A Heterozygous Mutation in Cardiac Troponin T Promotes Ca²⁺ Dysregulation and Adult Cardiomyopathy in Zebrafish

Other Figures

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Figure 4

Embryos of tnnt2a^+/RK94del show decreased heart rate accompanied by decreased ventricular diastolic and systolic volumes, as well as increased relaxation time. Side view and top view of 5 dpf (A) tnnt2a^+/+ and (B) tnnt2a^+/RK94del embryos. (C–F) Bar graph of contractility parameters, including heart rate, contraction time, relaxation time, and systolic fraction for tnnt2a^+/+ and tnnt2a^+/RK94del. (G) For hemodynamic parameters, we used ImageJ to fit an ellipse over the ventricle in every recording, both at end-diastole (left) and end-systole (right). (H–L) Bar graphs of hemodynamic parameters, including end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF), stroke volume (SV), and cardiac output (CO). (M–O) Bar graphs of surface area parameters: diastolic surface area, systolic diastolic surface area, and fractional area change (FAC). Scale bar: 100 µm. Statistics: mean ± SEM, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, tnnt2a^+/+ n = 19, tnnt2a^+/RK94del n = 24, unpaired Students t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tnnt2a^hu11320/+ (TL)
Observed In:	heart contraction whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Cardiovasc Dev Dis