Figure 6

Immunohistochemical and histochemical labelling of the zebrafish olfactory bulb. Transverse sections through the central area of the olfactory bulb showing immunoreactivity with the antibodies anti-CB, anti-CR, anti-LHRH, anti-Gαi2, anti-Gαo and LEA lectin labelling. Calcium-binding proteins (CB and CR) are both mainly expressed in the dorsal (dG), dorsolateral (dlG) and lateral (lGx, lG₁) glomeruli. Additionally, calbindin is also expressed in glomeruli belonging to the mediodorsal part of the bulb (mdG) and, with less intensity, in the ventral posterior area (VpG). LHRH is expressed in the dorsolateral glomeruli. Both anti G-proteins show a similar pattern, with an intense labelling in the ventromedial glomerular (VmG) area, stronger in the case of Gαo. Additionally, anti-Gαi2 marks glomeruli belonging to the ventral posterior cluster (VpG). LEA labelling is mainly circumscribed to the ventral region. DL, dorsolateral; VL, ventrolateral; dG, dorsal glomeruli; dlG, dorsolateral glomeruli; lG₁, lateral glomerulus 1; lGx, lateral cluster of glomeruli; mdG, mediodorsal glomeruli; VmG_1-6, ventromedial glomeruli 1–6; VmG_7, ventromedial glomerulus 7; VpG, ventral posterior glomeruli; d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: 100 µm.