FIGURE

Fig. 8

ID

ZDB-FIG-210413-55

Publication

Luo et al., 2020 - Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish

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Fig. 8

Temozolomide treatment inhibits gliomagenesis in nf1 KO and nf1;tp53 cKO fish until 3 months of age. (A) Mortality analyses of nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment (n = 100 for each group; two groups for each line). (B) Tumour incidences were significantly suppressed by TMZ treatment in nf1 KO and nf1;tp53 cKO fish at 3 months of age (n = 100 for each group; two groups for each line). (C) Representative immunohistochemistry images examining the expression of Pcna, pHH3, Gfap, Nestin, Cyclin D1, and pAkt in nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment. Scale bars = 100 μm. (D) Quantification of the percentages of Pcna-positive cells in nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment (n = 5 random fields of view). Data shown as mean ± SEM. *P < 0.05.

Expression Data

Genes:	ccnd1 gfap nes pcna
Fish:	AB nf1a^{zf3453a/zf3453a}; zf3453Tg (AB) nf1a^{zf3453a/zf3453a}; tp53^{zf3455a/zf3455a}; zf3453Tg; zf3455Tg
Condition:	chemical treatment by environment: temozolomide
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nf1a^{zf3453a/zf3453a}; zf3453Tg (AB) nf1a^{zf3453a/zf3453a}; tp53^{zf3455a/zf3455a}; zf3453Tg; zf3455Tg
Condition:	chemical treatment by environment: temozolomide
Observed In:	brain brain cell population proliferation brain glioblastoma whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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