Figure 3

Abnormal cartilage and bone formation in mafbb mutants. (A) Alcian Blue staining of embryos at 5 dpf. A1-A3, ventral views; A1?-A3?, lateral views. Ceratobranchial pairs (cb); ceratohyal (ch); Meckel?s cartilage (mk); basihyal (bh), CHA (ceratohyal angel). (B) Summary of CHA in embryos at 5 dpf (n = 15 embryos per group). (C) Ventral views of Alizarin Red (AR) staining of larvae at 8 dpf. C1? and C2? are the enlarged images of the teeth region (n = 30 embryos per group). (D) MicroCT scans of adult zebrafish at 10 mpf. The hypurals in the red boxed area are used for analysis in E. (E) Summary of BV/TV for the bones in the boxed area in D (n = 3 zebrafish per group); mean grey-level intensities of the boxed area in D. (F) AR staining of adult zebrafish at 9 mpf. F1??F3?? are higher magnifications of boxed area in F1?F3. Mouths (F?), vertebral columns (F?) and caudal fins (F??). The black arrows in F1??F3? point to the lower jaw; the black arrowheads in F2?-F3? point to the neural arches; the blue arrowheads in F1???F3?? point to hypurals 3?5 (H3?5); The blue arrows point to H1,2; N = 3 zebrafish per group. WT, wild type; HET, mafb^bd11/+; HOM, mafbb^d11/d11; BV, bone volume; TV, total tissue volume. Results in B and E are expressed as mean ± SEM, (* p < 0.05, **** p < 0.0001, t test).