FIGURE

Figure 7

ID

ZDB-FIG-210301-75

Publication

Atienzar-Aroca et al., 2021 - Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation

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Figure 7

Immunohistochemical detection of myoc in the reproductive system of adult zebrafish. Tissue sections (14 μm) of adult (7 months) wild-type ovary (A,B) and testis (C,D) and KO myoc testis (E,F) were incubated with an anti-myocilin (TNT) primary antibody, followed by Cy2-conjugate goat anti-chicken IgY secondary antibody (green signals). Note that ovaries from −/− animals were not available because of the absence of females with this genotype. White and yellow arrows indicate cortical granules and follicular epithelium-associated immunoreactivity, respectively. White arrowheads show immunolabeling in the seminiferous epithelium (C,D). Red signals correspond to tissue autofluorescence. The images are representative of the result observed in three tissue sections from three animals. +/+: wild-type; −/−: myoc KO. cg: cortical granules. fe: follicular epithelium. *: zona radiata. Negative controls are shown in Figure S6.

Expression Data

Antibody:	Ab1-myoc
Fish:	AB myoc^ucl2/ucl2 (AB)
Anatomical Terms:	oocyte ovarian follicle epithelium seminiferous epithelium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myoc^ucl2/ucl2 (AB)
Observed In:	seminiferous epithelium
Stage:	Adult

Phenotype Detail

Acknowledgments

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