FIGURE

Figure 6

ID

ZDB-FIG-210301-72

Publication

Atienzar-Aroca et al., 2021 - Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation

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Figure 6

Immunohistochemistry of myocilin in non-ocular tissues of adult zebrafish. Wild-type and myoc KO adult (7 months) zebrafish tissue sections (14 μm) were incubated with a chicken anti-myocilin (TNT) primary antibody and a Cy2-conjugate goat anti-chicken IgY secondary antibody. Arrows show immunostaining in the periphery of pharyngeal muscular fibres (A,B,G,H), the enterocyte apical side in the intestinal bulb (C,D,I,J) and in the brush border of the epithelial cells in the middle intestine (E,F,K,L). Red signals correspond to tissue autofluorescence. bb: brush border. The images are representative of the results observed in three tissue sections from three animals. +/+: wild-type; −/−: myoc KO. Negative controls are shown in Figure S5.

Expression Data

Antibody:	Ab1-myoc
Fish:	AB myoc^ucl2/ucl2 (AB)
Anatomical Terms:	intestinal bulb enterocyte intestine epithelial cell pharyngeal musculature peripheral region
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myoc^ucl2/ucl2 (AB)
Observed In:	intestinal bulb enterocyte intestine epithelial cell pharyngeal musculature peripheral region
Stage:	Adult

Phenotype Detail

Acknowledgments

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