Figure 6—figure supplement 2.

MEP glia do not exit the spinal cord in the absence of motor axons.

(A) In situ hybridization showing wif1+ MEP glia (outlined arrowheads) along the motor nerve root in a DMSO-treated hb9:yfp-ntr larva and wif1+ MEP glia (arrowheads) in the spinal cord of a MTZ-treated hb9:yfp-ntr larva at 3 dpf. (B) Lateral views of foxd3:mcherry;hb9:yfp-ntr larvae at 50 and 72 hpf, treated with either 2% DMSO or 20 mM metronidazole (MTZ)/2% DMSO from 9 to 72 hpf, showing foxd3+ MEP glia (outlined arrowheads) along motor axons in the control larvae. In the MTZ-treated larvae, foxd3+ MEP glia did not exit the spinal cord. Yellow dashed lines denote the edge of the spinal cord and asterisks denote the DRG. Scale bar, (A) 10 µm, (B) 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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