FIGURE

Fig. 5

ID

ZDB-FIG-210217-60

Publication

Turan et al., 2020 - The endosomal trafficking regulator LITAF controls the cardiac Nav1.5 channel via the ubiquitin ligase NEDD4-2

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Figure 5. LITAF down-regulates total NEDD4-2 expression. Adenovirus for expression of GFP (control) or LITAF-HA was incubated with 3wRbCM (10 MOI) (A) or NRbCM (2 MOI) (B) for 48 h. HEK cells were transfected with plasmid encoding GFP (control) or HA-tagged LITAF (C) for 48 h. Representative Western blotting data from respective extracts to measure expression of NEDD4-2, tubulin, and LITAF-HA. D, NEDD4-2–FLAG, LITAF-HA, and tubulin expression in HEK cells transfected with expression plasmids for NEDD4-2–FLAG, LITAF-HA, or control plasmid for 48 h. E, Respective relative LITAF-dependent NEDD4-2 expression levels normalized to tubulin expression from four independent experiments performed in duplicate (Student's t test; p < 0.05). In the scatter plot, averaged values for the four independent experiments together with the means and S.E. values are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.